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Video EditR CRISPRCas9 Gene Engineering Platform GE
CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes: Cell
Gene knockout workflow using the Edit-R Lentiviral Cas9 nuclease with lentiviral sgRNA
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Fernández A, Josa S, Montoliu L. Mamm Genome. 2017 Jun 6. doi: 10.1007/s00335-017-9699-2. --> Review on the brief but intense history of genome editing in ...
Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion
Commercial resources for CRISPR-Cas-related reagents.
Other sources of Cas9 for genome editing experiments.
Gene knockout workflow using the Edit-R Cas9 Nuclease mRNA with synthetic crRNA and tracrRNA
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CRISPR–Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome
In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering
Figure 1
Ectopic expression of RAD52 and dn53BP1 improves homology-directed repair during CRISPR–Cas9 genome editing
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How much crRNA & tracrRNA do I need?
Correction of a pathogenic gene mutation in human embryos
a, Schematic depicting wild-type SpCas9 interactions with the target DNA–sgRNA duplex, based on PDB accession 4OO8 and 4UN3 (adapted from refs 28 and 29, ...
Edit-R CRISPR CAS9 System - We just changed the way you edit genes
Inhibition of 53BP1 favors homology-dependent DNA repair and increases CRISPR–Cas9 genome-editing efficiency
CRISPR-GE: A Convenient Software Toolkit for CRISPR-Based Genome Editing
Simultaneous lineage tracing and cell-type identification using CRISPR–Cas9-induced genetic scars
p53 inhibits CRISPR–Cas9 engineering in human pluripotent stem cells
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Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR–Cas9 library
DNA, RNA, Protein— Let Me Introduce You with CRISPR/Cas9 | Biocompare: The Buyer's Guide for Life Scientists
The developmental potential of human embryos following CRISPR-Cas9-mediated genome editing. a
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Enriched guides do not display sequence bias and cluster to regions on the MS2 genome.
High-throughput in situ cell electroporation microsystem for parallel delivery of single guide RNAs into mammalian cells | Scientific Reports
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Review Article| Volume 9, ISSUE 7, P961-974, July 06, 2016. CRISPR/Cas9 Platforms for Genome ...
Locus-specific histone deacetylation using a synthetic CRISPR-Cas9-based HDAC
Repression of GFP mRNA.
SauCas9 repression of a GFP reporter in vivo.
ZFs, TALEs and CRISPR/Cas9 systems for genome editing and gene expression manipulation. ZFP, zinc-finger protein; FD, functional domain; TFs, ...
Review Article| Volume 11, ISSUE 3, P359-380, March 05, 2018
a, Schematic of the first cell division in human embryos and time of microinjection. PN, pronuclei; PNF, pronuclear fading. b, Representative human embryo ...
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Development and Applications of CRISPR-Cas9 for Genome Engineeri
In vitro RNA cleavage is impaired by strong secondary structure.
Screening sgRNAs targeting OCT4 in optimized inducible CRISPR-Cas9 knockout human ES cells and mouse
Enrichment of Cas9-expressing U2OS cells using SMARTCas9-mKate2 expression plasmid by FACS results
Mechanism for targeted gene disruption by the Cas9/sgRNA complex and subsequent mutagenesis by NHEJ
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SauCas9 cleaves single-stranded RNA without a PAMmer.
SauCas9 confers in vivo protection against an RNA phage.
RNA cleavage is limited by the RNA target.
poster:Using machine learning to identify the best CRISPR-Cas9 targets for functional gene
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Gene editing is dose-dependent when directed by the RNP and the ssODN. Synchronized
Development and Applications of CRISPR-Cas9 for Genome Engineeri
RNA is cleaved by SauCas9 and CjeCas9.
Reactivating Fetal Hemoglobin Expression in Human Adult Erythroblasts Through BCL11A Knockdown Using Targeted Endonucleases: Molecular Therapy - Nucleic ...
Next-Generation Plant Metabolic Engineering, Inspired by an Ancient Chinese Irrigation System: Molecular Plant
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(a) Schematic representation of the genome localization of c.156 C > A mutation in Vps16 gene and construction of the targeting sgRNA and oligonucleotide.
Transient CRISPR-Cas9 system. (A) Structures of CaCAS9 and sgRNA expression cassettes
How to identify key genes with CRISPR-Cas9 and shRNA screens
Confirmation that enriched guides from the MS2 screen confer protection against MS2 infection.
CRISPR permits simultaneous targeting of CDR1 and CDR2, which mediate resistance to fluconazole and cycloheximide
SauCas9 cleavage of different nucleic acid substrates.
a, Proportion of POU5F1-null, heterozygous or wild-type (WT) cells in each human embryo. The number of separate individual cells analysed is indicated.
Two-step genome engineering timeline. Schematic overview of the major steps of the genome
a, Principal component analysis of single-cell RNA-seq data showing comparisons between the cells from human blastocysts that developed following ...
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Development and Applications of CRISPR-Cas9 for Genome Engineeri
CRISPR-UMI: single-cell lineage tracing of pooled CRISPR–Cas9 screens
ssRNA cleavage is similar to canonical dsDNA cleavage by Cas9.
Figure 1
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SauCas9 prefers a complementary region of 23nt for binding and cleavage.
Gene knockout workflow using the Edit-R Inducible Lentiviral Cas9 Nuclease in a pooled lentiviral
Development and Applications of CRISPR-Cas9 for Genome Engineeri